> Alan S. L. Yu, M.D.

Dr. Yu graduated from Cambridge University, England and obtained his postgraduate training at the Brigham and Women’s Hospital and Harvard Medical School. In 2002, he joined the faculty at the USC Keck School of Medicine, where he is Assistant Professor in the Division of Nephrology and in the Department of Physiology and Biophysics. Dr. Yu has an active NIH-funded laboratory research program, focused on elucidating the molecular and structural basis of renal tubule epithelial transport. His laboratory utilizes state-of-the-art techniques, including molecular and cell biology, proteomics, electrophysiology and transgenic mouse models. Investigation of renal tubule physiology may reveal novel insights into the cause of a variety of kidney diseases including acute renal failure and hypertension.

Curriculum Vitae

A. Personal Information :

B. Education :

C. Professional Background :

Academic Appointments

Assistant Professor of Medicine, Keck School of Medicine, University of Southern California , Los Angeles , CA , 2002–Present

Assistant Professor of Physiology and Biophysics, Keck School of Medicine, University of Southern California , Los Angeles , CA , 2002–Present

Assistant Professor of Medicine, Harvard Medical School , Boston, MA, 2000–2002

Instructor in Medicine, Harvard Medical School , Boston, MA, 1995–2000

Specific Teaching Responsibilities

Lecturer and course instructor for Physiology Department graduate course on “Ion Channels and Disease” (PHBI 650), 2004

Lecturer and workshop leader for medical student Year II Renal Organ Systems course, 2002-Present

Lecturer, weekly renal fellow lecture series, 2002-Present

Lecturer, Medical Grand Rounds for internal medicine housestaff, 2002-Present

Facilitator, renal tutorials for medical students in internal medicine at LAC-USC, 2002-Present

Clinical Administrative Appointments

Assistant Director of Dialysis, Brigham and Women's Hospital, Boston, MA, 2001–2002

Associate Section Chief, West Roxbury Veterans Administration Hospital , Boston, MA, 2000–2001

Other

Medical Staff, LAC+ USC Medical Center , USC University Hospital , and USC/Norris Comprehensive Cancer Center and Hospital, Los Angeles , CA , 2002–Present

Consultant Staff, Dana-Farber Cancer Institute, Boston, MA, 1998–2002

Staff Physician, West Roxbury Veterans Administration Hospital , Boston, MA, 1998–2002

Associate Physician, Brigham and Women's Hospital, Boston, MA, 1996–2002

D. Society Memberships :

Professional

National Kidney Foundation, 1991–Present

American Society of Nephrology, 1993–Present

Salt and Water Club, Member (elected), 1997–2002

Massachusetts Medical Society, 1999–2002

American Physiological Society, 2001–Present

West Coast Salt and Water Club, Council Member (elected), 2004

E. Service :

University/Other Committees

Internship Selection Committee, Department of Medicine, Brigham and Women's Hospital, 1999–2002

American Physiological Society, Renal Section Awards Committee, 2004–Present

Editorships

Section Editor, Current Opinion in Nephrology and Hypertension, 2000–2003

Editorial Boards

Journal of the American Society of Nephrology, 2003–Present

American Journal of Physiology: Renal Physiology, 2003–Present

Evaluation Board, Faculty of 1000 Medicine, 2004–Present

Grant Reviews

External reviewer, Wellcome Trust Programme Grants ( UK ), 2000–Present

External Referee Panel, National Kidney Research Fund ( UK ), 2001–Present

American Heart Association, Western Review Consortium Committee, 2003

Ad hoc member, NIH General Medicine B Study Section, 10/2003

Ad hoc member , Veterans Administration Nephrology Merit Review Subcommittee, 11/2004

Advisory Boards

Acute Renal Failure Medical Advisory Board, Genzyme Corp., 2005

F. Research Activities :

Major Areas of Research Interest

Epithelial transport, renal tubule function, paracellular transport, tight junctions

Research Grants

G. Invited Lectures :

International

Molecular characterization of renal calcium channels. 12 th International Congress of Nephrology, Jerusalem , Israel , 6/1993.

Other

Research Seminar, Renal Division, Toronto General Hospital , Toronto , ON , Canada , 12/1995.

Workshop Leader, Postgraduate Education Courses in Molecular Biology, American Society of Nephrology, 1996–1999.

Research talk, Salt and Water Club, Yale University , New Haven , CT , 9/1997.

Invited Lecture, Clinical Pharmacology Unit, Smith-Kline Beecham Pharmaceuticals, Philadelphia , PA , 3/1999.

Epithelial Biology Series Lecture, Departments of Medicine and Physiology, Yale University , New Haven , CT , 12/1999.

Visiting Professor, Department of Physiology, University of Texas Medical Branch at Galveston , TX , 1/2000.

Research Seminar, Renal Division, University of Texas Southwestern Medical School , Dallas , TX , 5/2000.

Research Seminar, Renal Division, University of Texas , Houston , TX , 7/2000.

Research Seminar, Division of Nephrology, University of Colorado, Denver, CO, 10/2000.

Research Seminar, Division of Nephrology, University of Washington , Seattle , WA , 1/2001.

Visiting Professor, Division of Mineral Metabolism, University of Texas Southwestern Medical School , Dallas , TX , 4/2001.

Nephrology Research Training Center Lecture, University of Alabama , Birmingham , AL , 6/2001.

Research Seminar, Division of Nephrology, Oregon Health Sciences University , Portland , OR , 6/2001.

Renal Research Conference, University of Pittsburgh , Pittsburgh , PA , 9/2001.

Visiting Professor, Kidney and Electrolyte Physiology Section, NHLBI, NIH, Bethesda , MD , 10/2001.

Research Seminar, Division of Nephrology, University of Maryland , Baltimore , MD , 12/2001.

Workshop Leader, American Society of Nephrology Postgraduate Education Course on Advances in Cell and Molecular Biology, Philadelphia , PA , 10/2002.

Moderator, Symposium on Chloride Channels, American Society of Nephrology Annual Meeting, Philadelphia , PA , 11/2002.

Research Seminar, Division of Nephrology, Stanford University , Palo Alto , CA , 11/2002.

Research Seminar, Division of Nephrology, University of California , San Francisco , CA , 11/2002.

Research Seminar, Division of Nephrology, Northwestern University, Evanston , IL , 1/2003.

Research Seminar, Division of Nephrology and Hypertension, Harbor-UCLA Medical Center , Los Angeles , CA , 1/2004.

Research Seminar, Division of Nephrology, Stanford University , Palo Alto , CA , 1/2004.

Research Seminar, Division of Nephrology, UCLA Medical Center , Los Angeles , CA , 2/2004.

Invited Symposium Lecture, FASEB Experimental Biology Annual Meeting, Washington , DC , 4/2004.

Claudins and Paracellular Transport, Invited Lecture at American Society of Nephrology Annual Meeting, St. Louis , MO , 10/2004.

Research Seminar, Division of Nephrology, University of California , San Francisco , CA , 11/2004.

Invited Lecture, Coordinating Hemodynamic, Filtration, and Reabsorptive Functions of the Kidney. Satellite Symposium, XXXV International Congress of Physiological Sciences/FASEB Meeting, San Diego , CA , 3/2005.

H. Bibliography :

Peer Reviewed

1. Heilig CW, Brenner RM, Yu ASL , Kone BC , Gullans SR: Modulation of osmolytes in MDCK cells by solutes, inhibitors and vasopressin. Am J Physiol Renal Physiol 259:F653–659, 1990.

2. Yu ASL , Hebert SC , Brenner BM, Lytton J: Molecular characterization and nephron distribution of the pore-forming subunit of a family of renal Ca 2+ channels. Proc Natl Acad Sci USA 89:10494–10498, 1992.

3. Yu ASL , Hebert SC, Lee SL, Brenner BM, Lytton J: Identification and localization of the renal Na + -Ca 2+ exchanger by the polymerase chain reaction. Am J Physiol Renal Physiol 263:F680–F685, 1992.

4. Lee SL, Yu ASL , Lytton J: Tissue specific expression of Na + -Ca 2+ exchanger isoforms. J Biol Chem 269:14849–14852, 1994.

5. Yu ASL , Boim M, Hebert SC , Castellano A, Perez-Reyes E, Lytton J: Molecular characterization of renal calcium channel b subunit transcripts. Am J Physiol Renal Physiol 268: F525–F531, 1995.

•  Van Baal J, Yu ASL , Hartog A, Fransen JAM, Willems PHGM, Lytton J, Bindels RJM: Localization and regulation by vitamin D of calcium transport proteins in rabbit cortical collecting system. Am J Physiol Renal Physiol 271:F985–F993, 1996.

•  Yu ASL : Atypical electrocardiographic changes in severe hyperkalemia. Am J Cardiol 77:906–908, 1996.

•  Yu ASL , Levy E: Paradoxical cerebral air embolism from a hemodialysis catheter. Am J Kidney Dis 29:453–455, 1997.

9. Barry ELR, Gesek FA, Yu ASL , Lytton J, Friedman PA: Distinct calcium channel isoforms mediate parathyroid hormone and chlorothiazide-stimulated calcium entry in transporting epithelial cells. J Membr Biol 161:55–64, 1998.

10. Luyckx VA, Goda FO, Mount DB, Nishio T, Hall A, Hebert SC, Hammond TG, Yu ASL : Intrarenal and subcellular localization of rat CLC5. Am J Physiol Renal Physiol 275:F761–F769, 1998.

11. Luyckx VA, Leclercq B, Dowland L, Yu ASL : Diet-dependent hypercalciuria in transgenic mice with reduced CLC5 chloride channel expression. Proc Natl Acad Sci USA 96:12174–12179, 1999.

12. Pavlova A, Sakurai H, Leclercq B, Beier DR , Yu ASL , Nigam SK : Developmentally regulated expression of the organic ion transporters, NKT/mOAT, OCT-1, NLT and roct during murine embryogenesis. Am J Physiol Renal Physiol 278:F635–F643, 2000.

13. Wills NK, Weng T, Mo L, Hellmich HL, Yu ASL , Wang T, Bucheit S, Godley BF: Chloride channel expression in cultured human fetal RPE cells: Response to oxidative stress. Invest Ophthalm Vis Sci 41:4247–4255, 2000.

14. Dowland LK, Luyckx VA , Enck AH, Leclercq B, Yu ASL : Molecular cloning and characterization of an intracellular chloride channel in the proximal tubule cell line, LLC-PK1. J Biol Chem 275:37765–37773, 2000.

15. Weng TX, Mo L, Hellmich HL, Yu ASL , Wang T, Wills NK: Expression and regulation of ClC-5 chloride channels: Effects of antisense and oxidants. Am J Physiol Cell Physiol 280:C1511–1520, 2001.

16. Enck AH, Berger U, Yu ASL : Claudin-2 is selectively expressed in the proximal nephron in mouse kidney. Am J Physiol Renal Physiol 281: F966–974, 2001.

17. Weng TX, Godley BF, Jin GF, Mangini NJ , Kennedy BG, Yu ASL , Wills NK: Chloride channels expressed in human adult retina. Am J Physiol Cell Physiol 283:C839–C849, 2002.

18. Isnard-Bagnis C, Da Silva N, Beaulieu V, Yu ASL , Brown D, Breton S: Detection of the chloride channels ClC-3 and ClC-5 in epithelial cells of the epididymis by immunofluorescence and RT-PCR after laser capture microdissection. Am J Physiol Cell Physiol 284:C220–C232, 2003.

19. Yu ASL , Enck AH, Lencer WI , Schneeberger EE: Functional expression of claudin-8 reveals a role in the paracellular cation barrier. J Biol Chem 278:17350–17359, 2003.

•  Li WY , Huey CL, Yu ASL . Expression of claudins 7 and 8 along the mouse nephron. Am J Physiol Renal Physiol 286:F1063-F1071, 2004.

•  Huey CL, Riepe FG, Sippell WG, Yu ASL . Genetic heterogeneity in autosomal dominant pseudohypoaldosteronism type I: Exclusion of claudin-8 as a candidate gene. Am J Nephrol 24:483-487, 2004.

•  Yu ASL , McCarthy KM, Francis SA, McCormack JM, Lynch RD , Schneeberger EE. Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells. Am J Physiol Cell Physiol , in press.

Non Peer Reviewed

1. Brenner BM, Yu ASL : Uremic syndrome revisited: A pathogenetic role for retained endogenous inhibitors of nitric oxide synthesis. Curr Opin Nephrol Hypertens 1:3–7, 1992.

2. Yu ASL : Calcium channels. Curr Opin Nephrol Hypertens 3:497–503, 1994.

3. Yu ASL : Identification and localization of calcium channel a 1 and b subunit isoforms in the kidney. Kidney Int 48:1097–1101, 1995.

4. Yu ASL : Paracellular solute transport: more than just a leak? Curr Opin Nephrol Hypertens 9:513–515, 2000.

5. Yu ASL : Role of ClC-5 in the pathogenesis of hypercalciuria: recent insights from transgenic mouse models. Curr Opin Nephrol Hypertens 10:415–420, 2001.

6. Yu ASL : Evolving concepts in epithelial magnesium transport. Curr Opin Nephrol Hypertens 10:649–653, 2001.

7. Yu ASL : Elucidating the structural basis of membrane transport protein function: Light at the end of the channel? Curr Opin Nephrol Hypertens 11:523–526, 2002.

Abstracts (selected)

1. Dowland LK, Luyckx VA , Enck AH, Leclercq B, Yu ASL : Molecular cloning and characterization of an intracellular chloride channel in the proximal tubule cell line, LLC-PK1 J Am Soc Nephrol 11:27A, 2000.

2. Bagnis C, Nsumu NN, Yu ASL , Brown D, Breton S: Immunolocalization of chloride channels ClC-3, 4 and 5 in rat epididymis J Am Soc Nephrol 11:25A, 2000.

3. Yu ASL , Enck AH, Berger U: Claudin-2 is selectively expressed in the proximal nephron in mouse kidney. J Am Soc Nephrol 12:45A, 2001.

4. Yu ASL , Enck AH: Claudin-8 mRNA expression in tight epithelia of the distal renal tubule. J Am Soc Nephrol 12:45A, 2001.

5. Wagner CA, Enck AH, Giebisch GH, Yu ASL , Geibel JP: Normal H+-ATPase function in the proximal tubule and collecting duct in a mouse model for Dent´s disease. J Am Soc Nephrol 12:11A, 2001.

•  Yu ASL , Enck AH: The distal tubule tight junction protein, claudin-8, contributes to the paracellular barrier to charged solutes. J Am Soc Nephrol 13:488A, 2002.

•  Yu ASL , Li, WY, Sandoval B, Yang LE, Leong PKK: Claudin-8 is expressed in tight junctions of the aldosterone-sensitive distal nephron and trafficks to the basal membrane in acute hypertension. J Am Soc Nephrol 14:83A, 2003.

Abstracts Submitted

•  Ahlstrom RE, Yu ASL . Serine-threonine kinase activity of wild type and mutant WNK4. International Congress of Physiological Sciences Meeting , Submitted 2005.

Chapters

1. Yu ASL : Calcium channels. In: Molecular Nephrology: Kidney Function in Health and Disease (Bonventre JV, Schlondorff D [editors]). Marcel Dekker, New York , NY , pp427–438, 1995.

2. Yu ASL : Disturbances of magnesium metabolism. In: Brenner and Rector's The Kidney (6 th edition; Brenner BM [editor]). Saunders, Philadelphia , PA , pp1055–1070, 2000.

3. Yu ASL , Brenner BM: Tubulointerstitial diseases of the kidney. In: Harrisons Principles of Internal Medicine (15 th edition; Braunwald E, et al [editors]). McGraw-Hill , New York , NY, pp1606–1610, 2001.

4. Smirnakis KV, Yu ASL : Diuretics in acute renal failure. In: Therapy of Nephrology and Hypertension (2 nd edition; Wilcox C, Brady HR [editors]). Saunders, Philadelphia , PA , pp33-37, 2003.

6. Yu ASL : Renal transport of calcium, magnesium and phosphate. In: Brenner and Rector's The Kidney (7 th edition; Brenner BM [editor]). Saunders, Philadelphia , PA , Vol. I., pp535-571, 2004.

7. Pollak M, Yu ASL : Clinical disturbances of calcium, magnesium and phosphate metabolism. In: Brenner and Rector's The Kidney (7 th edition; Brenner BM [editor]). Saunders, Philadelphia , PA , pp 1041-1076, 2004.

8. Yu ASL , Brenner BM: Tubulointerstitial diseases of the kidney. In: Harrisons Principles of Internal Medicine (16 th edition; Kasper DL, et al [editors]). McGraw-Hill , New York , NY, pp 1702-1706, 2005.

Chapters in preparation

1. Mount D , Yu ASL : Renal transport of inorganic solutes. In: Brenner and Rector's The Kidney (8 th edition; Brenner BM [editor]). Saunders, Philadelphia , PA.